An <i>ex-vivo</i> study to investigate the potential of <i>Staphylococcus epidermidis</i> lysate to improve wound healing in diabetic patients

Appropriate treatment of non-healing diabetic foot ulcers is challenging as the whole skin repair process complicated. Current therapeutic strategies have to be evolved for better efficacy. Staphylococcus epidermidis (S. epidermidis) a commensal having probiotic properties. The wound healing potential its lysate was explored using biopsies. Full-thickness skin, obtained from individuals with and without diabetes, wounded an excisional punch. Skin explants were cultured serum-free medium in presence absence S. lysate. ability stimulate re-epithelialisation assessed ex-vivo human model wounding patients diabetes. Epithelial tongue length significantly reduced wounds comparison non-diabetic samples, even after 12 days post-wounding. Re-epithelialisation augmented considerably lysate-treated ex-vivo. In increase twofold greater but only slight enhancement observed control (untreated sample). epidermal thickness also increased 7.7-fold treated 4.4-fold untreated samples. cell number per unit area showed significant Ki-67 positive cells slighter indicating expression proliferation marker newly formed epithelial samples (both non-diabetic). promotes both biopsies may used accelerate chronic ulcer wounds. Its needs further vivo molecular studies.